DESCRIPTION

Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif, the pentraxin domain. Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties. Pentraxin 3 is an acute phase glycoprotein that plays a role in the regulation of innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility and cancer. Pentraxin 3 is a biomarker for cardiovascular disease. Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	585.6	23.7	4.0
2	8	163.7	8.1	4.9
3	8	87.6	3.1	3.6

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	642.4	63.2	9.8
2	16	161.6	9.3	5.8
3	16	84.4	6.6	7.8

RECOVERY

The recovery of human Pentraxin 3/TSG-14 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:16	109	94-127
	1:32	106	94-120
Saliva	1:8	99	91-111
	1:16	106	95-122
Cell lysate	1:512	90	75-100

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:2. The cell lysate was initially diluted 1:32. )

		Human serum(Sample Diluent PT 4B1)	Saliva(Sample Diluent PT 3)	Cell lysate(Sample Diluent PT 4B1)
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	102	105	101
	Range (%)	99-106	97-112	100-102
1:8	Average% of Expected	103	98	109
	Range (%)	102-103	91-106	103-114
1:16	Average% of Expected	109	105	122
	Range (%)	100-117	96-113	117-127

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human Pentraxin 3/TSG-14 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)