DESCRIPTION

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redoxsensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	20	13.10	0.40	3.06
2	20	2.94	0.07	2.24
3	20	0.76	0.04	4.85

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	24	13.25	1.04	7.88
2	24	2.84	0.19	6.54
3	24	0.75	0.07	9.68

RECOVERY

The recovery of human PARK7/DJ-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:16	118	112-124
	1:32	91	81-103
Cell lysate	1:3,200	93	83-102
	1:6,400	95	85-104
Human cerebrospinal fluid (CSF)	1:2	98	83-119
	1:4	95	87-98

LINEARITY

To assess the linearity of the assay, human serum, cell lysate and human cerebrospinal fluid (CSF) samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:2. The cell lysate was initially diluted 1:400.)

		Human serum (SampleDiluent PT 3)	Cell lysate (SampleDiluent PT 1)	CSF (Sample Diluent PT1)
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	108	98	106
	Range (%)	104-111	97-99	102-111
1:8	Average% of Expected	110	106	98
	Range (%)	107-112	105-107	85-110
1:16	Average% of Expected	95	113	119
	Range (%)	86-106	109-116	114-124

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PARK7/DJ-1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)