DESCRIPTION

Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	272.4	12.5	4.6
2	20	61.4	0.6	1.0
3	20	14.9	0.3	2.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	274.0	17.6	6.4
2	24	61.8	2.6	4.3
3	24	15.7	0.4	2.6

RECOVERY

The recovery of human MCP-1/CCL2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:16	92	89-95
	1:32	99	97-102
Cell culture supernatant	1:640	100	98-101
	1:1,280	103	99-107
Urine	1:16	90	89-91
	1:32	95	90-98

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:4. The cell culture supernatant was initially diluted 1:320. The urine was initially diluted 1:4. )

		Human serum	Cell culture supernatant	Urine
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	103	110	104
	Range (%)	100-106	104-116	99-109
1:8	Average% of Expected	102	103	107
	Range (%)	97-106	100-105	100-114
1:16	Average% of Expected	101	110	118
	Range (%)	96-107	96-125	116-121

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human MCP-1/CCL2 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)