DESCRIPTION

CD62L (L-selectin) is an adhesion molecule belonging to the selectin family, primarily expressed on the surface of white blood cells, and plays a role in various physiological and pathological processes, including lymphocyte homing, inflammatory responses, and immune cell positioning. It plays a crucial role in the homing process of white blood cells, helping them migrate from the bloodstream into lymphoid tissues. During inflammatory responses, CD62L mediates the initial adhesion of white blood cells to endothelial cells, facilitating their rolling and migration. Additionally, CD62L is a marker for central memory T cells, which have higher expression levels of CD62L and can rapidly relocate to lymph nodes and other sites for a swift secondary response.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	4.78	0.41	8.54
2	8	1.19	0.10	8.60
3	8	0.65	0.03	3.99

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	4.63	0.43	9.26
2	16	1.19	0.08	6.69
3	16	0.65	0.02	3.21

RECOVERY

The recovery of human CD62L spiked to three different levels throughout the range of the assay in human serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:800	105	92-124

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:50.)

		Human serum
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	126
	Range (%)	124-127
1:8	Average% of Expected	120
	Range (%)	119-121
1:16	Average% of Expected	112
	Range (%)	107-118

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human CD62L produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)