Product Name	Recombinant Rabbit Anti-NONO Monoclonal Antibody
Cat No.	SLA1938M
Chinese Name	核RNA结合蛋白P54重组兔单抗
Alias	MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114; nonA; CR201_G0029161; NONO_HUMAN; NONO; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein (p54(nrb) | p54nrb); 55 kDa nuclear protein (NMT55); DNA-binding p52/p100 complex, 52 kDa subunit; NONO_MOUSE; NONO_PONAB; NONO_RAT; non-POU domain containing octamer binding; non-POU-domain-containing, octamer-binding; Nuclear RNA-binding protein, 54-kD; non-Pou domain-containing octamer (ATGCAAAT) binding protein; protein phosphatase 1, regulatory subunit 114
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6D5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human NONO
Purification	affinity purified by Protein A
Predicted MW.	54 kDa
Observed MW.	63 kDa
Subcellular Location	N/A
Gene ID	4841
Swiss-Prot	Q15233
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	DNA- and RNA binding protein, involved in several nuclear processes.Binds the conventional octamer sequence in double-stranded DNA.Also binds single-stranded DNA and RNA at a site independent of the duplex site.Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with NMT55 monoclonal antibody, unconjugated (SLA1938M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 9. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 10. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 12. Paraformaldehyde-fixed, paraffin embedded Mouse Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 13. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NMT55 Monoclonal Antibody, Unconjugated (SLA1938M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 14. The A673 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NMT55 antibody (SLA1938M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.