Escherichia coli Quantitative Strain - Basic Information

Recommended Medium	Nutrient Agar/Nutrient Broth (NA/NB): beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 20.0g (liquid medium does not contain agar), distilled water 1.0L, pH 7.0. Sterilize at 121°C for 15 min. [Note] When culturing Bacillus, adding 5mg MnSO₄·H₂O is conducive to spore formation.
Growth Conditions	37°C; 18-24h; Aerobic
Storage Conditions	-20°C
Morphology	Colony diameter 1-2mm, round, regular margin, opaque, yellow on the front, lighter in color, convex center, smooth surface, easy to pick, G⁻ (red), rod-shaped
Procedure	① First culture the strain into a bacterial suspension according to the culture conditions; ② Measure the absorbance of the bacterial suspension, and calculate the initial concentration of the bacterial suspension according to the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension according to the actual concentration after lyophilization to obtain the target concentration, then aliquot and lyophilize.
Reference Use	Research; Analysis and detection. Strains for research and quality control
Sharing Method	Public welfare sharing