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Aspergillus brasiliensis Quantitative Strain - Basic Information
Recommended Medium Potato infusion powder: 10.0g, Glucose: 20.0g, KH2PO4: 3.0g, MgSO4·7H2O: 1.5g, Thiamine: 0.008g, Agar: 15.0g, pH: 6.0±0.2 (25℃) Growth Conditions 28℃;
7 days;
AerobicStorage Conditions -20℃ Morphology Small filamentous fungus, colonies are conspicuous on comprehensive PDA Recommended Medium. The plate mycelial layer is initially white, gradually turning black, and black spores are produced in the later stage. The reverse side of the Recommended Medium is brownish-black. Procedure
① First culture the strain into a bacterial suspension according to the culture conditions;
② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula;
③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization;
④ Dilute the bacterial suspension according to the actual concentration after lyophilization to obtain the target concentration, then aliquot and lyophilize;Reference Use GB 4789.28 Quality Requirements for Recommended Medium and Reagents Sharing Method Public Welfare Sharing Ordering & shipping
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