Candida albicans Quantitative Strain - Basic Information

Recommended Medium	Yeast extract powder: 3.0g, Malt extract: 3.0g, Glucose: 10.0g, Casein peptone: 5.0g, Agar: 20.0g, pH: 6.2±0.2 (25°C)
Growth Conditions	30°C; 24-48h; Aerobic
Storage Conditions	-20°C
Morphology	Round, regular margin, milky white front, light color, convex center, smooth surface, bright surface, moist texture, easy to pick, G+ (blue-purple), cocci, purity: pure
Isolation Source	Human blood, Bethesda, MD
Procedure	① First culture the strain into a bacterial suspension according to the culture conditions; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension according to the actual concentration after lyophilization to obtain the target concentration, then aliquot and lyophilize;
Reference Use	Strain used for testing the sterilization effect of medical devices in the "Technical Standard for Disinfection", positive control strain for "GB 4789.15-2010 Mold and Yeast Count", quality control strain in the 2015 edition of the Chinese Pharmacopoeia. Microorganisms in public articles and appliances, GB/T 18204.5-2013 Examination methods for public places - Part 5: Central air conditioning ventilation system, GB 4789.15-2016 National Food Safety Standard - Food Microbiological Examination - Mold and Yeast Count, GB 4789.28-2013 National Food Safety Standard - Food Microbiological Examination - Quality Requirements for Recommended Medium and Reagents; Positive control strain for mold and yeast examination methods in the "Technical Specification for Cosmetics".
Sharing Method	Public welfare sharing

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