Stbl3 (containing pLVX-TRE3G-mCherry plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃； 18-24h； Aerobic
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (mold 30℃, bacteria 37℃) and shake rapidly to dissolve； ② Disinfect the outer wall of the cryovial with an alcohol cotton pad, then transfer it to a biosafety cabinet for handling； ③ Unscrew the cap, aspirate all the dissolved liquid, and spread it onto 2 corresponding antibiotic plates, about 200 μL per plate； ④ Incubate the antibiotic plates under the specified conditions above for 18-24h, then observe (do not seal or wrap the plates)； ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic Recommended Medium, culture overnight at 37℃ on a shaker； ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction；
Sharing Method	Public Welfare Sharing