Stbl3 (Containing pscAAV8-GFP Plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold: 30°C, bacteria: 37°C) with rapid shaking until dissolved; ② Disinfect the outer wall of the cryovial with an alcohol cotton pad, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the thawed liquid, and dispense onto 2 corresponding antibiotic-resistant plates, approximately 200μL per plate; ④ Incubate the antibiotic-resistant plates under the above specified conditions for 18-24h before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37°C on a shaker; ⑥ According to experimental requirements, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing