DH5α (containing pACRISPR-BsaI plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (molds at 30°C, bacteria at 37°C) and shake rapidly to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol cotton pad, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all of the dissolved liquid, and dispense it onto 2 corresponding resistance plates, approximately 200 μL per plate; ④ Incubate the resistance plates under the specified conditions above for 18-24 h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB resistance Recommended Medium, and culture overnight at 37°C on a shaker; ⑥ According to experimental needs, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing

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