DH5α (containing pGL3-U6-sgRNA-PGK-mCherry plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (mold at 30℃, bacteria at 37℃), and shake rapidly to dissolve; ② Wipe the outer surface of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the dissolved liquid, and inoculate onto 2 corresponding antibiotic-resistant plates, approximately 200μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24h before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37℃ on a shaker; ⑥ According to experimental needs, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing