DH5α (containing pCMV-TurboID-MCS-FLAG-Neo plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold: 30°C, bacteria: 37°C), shaking rapidly until dissolved; ② Wipe the outer wall of the cryovial with an alcohol cotton pad for disinfection, then transfer to a biosafety cabinet for further handling; ③ Unscrew the cap, draw out all the dissolved liquid, and spread onto 1-2 agar plates (approximately 200μL per plate; for anaerobic cultures, the Recommended Medium should be placed in an anaerobic environment 24h in advance to remove oxygen); ④ Spread evenly and incubate under the culture conditions described above.
Sharing Method	Public Welfare Sharing