DH5α (containing pT3-EF1a-Cre-P2A-EGFP plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (molds at 30°C, bacteria at 37°C) with rapid shaking until dissolved; ② Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap and pipette out all the dissolved contents, then inoculate onto 1-2 agar plates (approximately 200 μL per plate; for anaerobic bacteria, place the Recommended Medium in an anaerobic environment for 24 h to remove oxygen beforehand); ④ Spread evenly and incubate under the growth conditions described above.
Sharing Method	Public Welfare Sharing