DH5α (containing pCAG-CBE4max-SpRY-CBE6b-EGFP plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C； 18-24 hours； Aerobic；
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold at 30°C, bacteria at 37°C), shaking rapidly to dissolve； ② Disinfect the outer wall of the cryovial with an alcohol wipe, then transfer to a biosafety cabinet； ③ Unscrew the cap, aspirate all the dissolved liquid, and spread onto 2 corresponding antibiotic plates, approximately 200 μL per plate； ④ Incubate the antibiotic plates under the above specified conditions for 18-24 h before observation (do not seal or wrap the plates)； ⑤ Pick a single colony and inoculate into 50 mL of liquid LB antibiotic Recommended Medium, culture overnight at 37°C on a shaker； ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction；
Sharing Method	Public Welfare Sharing

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