DH5α (Containing pCMV-T7-EGFP-MCS-3×FLAG-WPRE-Neo Plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 hours; Aerobic;
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (30°C for fungi, 37°C for bacteria) with rapid shaking to dissolve; ② Wipe the exterior of the cryovial with an alcohol swab to disinfect, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire dissolved solution, and dispense onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the conditions specified above for 18-24 h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50 mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37°C on a shaker; ⑥ Based on experimental requirements, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing

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