Escherichia coli BL21(DE3)(pET-32a(+)-Proteinase_3CL-pro)
BL21(DE3)（含pET-32a(+)-Proteinase-3CL-pro质粒）大肠杆菌
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BL21(DE3) (containing pET-32a(+)-Proteinase-3CL-pro plasmid) E. coli - Basic Information
Recommended Medium Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃) Growth Conditions 37℃；
18-24h；
Aerobic；Storage Conditions -80℃ Procedure
① Thaw the cryovial in a water bath (37℃) with rapid shaking until dissolved;
② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling;
③ Unscrew the cap and aspirate all the dissolved liquid, then dispense onto 1-2 agar plates (about 200 μL per plate);
④ Spread evenly and incubate under the culture conditions described above.Reference Use Plasmid extraction of pET-32a(+)-Proteinase_3CL-pro. Gene species: SARS-CoV-2 Sharing Method Public welfare sharing Ordering & shipping
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Partial purchase records(bought amounts latest6)
| Username | Quantity | bought time |
| Na*** | 1 | 2026-08-24 |
| Ge*** | 1 | 2026-08-09 |
| Qu*** | 1 | 2026-07-27 |
| Ia*** | 1 | 2026-07-21 |
| Is*** | 1 | 2026-06-12 |
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