DH5α (containing PKK232-8 plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C； 18-24h； Aerobic；
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (37°C) and shake rapidly to dissolve; ② Disinfect the exterior of the cryovial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap and aspirate all the dissolved liquid, then dispense onto 1-2 agar plates (approximately 200μL per plate); ④ Spread evenly and incubate under the above culture conditions.
Intended Use	Extraction of PKK232-8 plasmid.
Sharing Method	Public welfare sharing