BL21(DE3) (containing pET-32a(+)-NSP8 plasmid) Escherichia coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃； 18-24h； Aerobic；
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (37℃) and shake rapidly to dissolve; ② Wipe the outside of the cryovial with an alcohol cotton ball for disinfection, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, draw out all the dissolved liquid, and inoculate onto 1-2 agar plates (about 200μL per plate); ④ Spread evenly and then culture under the above growth conditions.
Reference Use	pET-32a(+)-NSP8 plasmid extraction. Gene species: SARS-CoV-2
Sharing Method	Public welfare sharing