BL21(DE3) (containing pET-32a(+)-NSP16 plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic;
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (37°C) and shake rapidly to dissolve; ② Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, pipette out all the dissolved liquid, and plate it onto 1-2 agar plates (about 200 μL per plate); ④ Spread evenly and incubate under the growth conditions described above.
Intended Use	Extraction of pET-32a(+)-NSP16 plasmid. Gene species: SARS-CoV-2
Sharing Method	Public welfare sharing