DH5α (containing pYEplac195-GAP-MCS plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	-80℃
Operating Procedures	① Thaw the cryovial in a water bath (molds at 30℃, bacteria at 37℃) and shake rapidly to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol cotton swab, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the dissolved liquid, and inoculate onto 1-2 agar plates (approximately 200μL per plate; for anaerobic bacteria, the Recommended Medium should be placed in an anaerobic environment for 24h in advance to remove oxygen); ④ Spread evenly and transfer to the above growth conditions for cultivation.
Sharing Method	Public Welfare Sharing