Escherichia coli DH5α(pcg 2.0-BFP-circ-arRNA backbone)
DH5α（含pcg 2.0-BFP-circ-arRNA backbone质粒）大肠杆菌
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DH5α (containing pcg 2.0-BFP-circ-arRNA backbone plasmid) E. coli - Basic Information
Recommended Medium Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃) Growth Conditions 37℃;
18-24h;
AerobicStorage Conditions -80℃ Procedure
① Thaw the cryovial in a water bath (mold at 30℃, bacteria at 37℃) and shake rapidly to dissolve;
② Wipe the outside of the cryovial with an alcohol swab to disinfect, then transfer to a biosafety cabinet for handling;
③ Unscrew the cap, pipette out all the dissolved liquid, and inoculate onto 1-2 agar plates (approximately 200μL per plate; for anaerobic bacteria, the Recommended Medium should be placed in an anaerobic environment for 24h to deoxygenate beforehand);
④ Spread evenly and incubate under the growth conditions described above.Sharing Method Public Welfare Sharing Ordering & shipping
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Partial purchase records(bought amounts latest3)
| Username | Quantity | bought time |
| Vi*** | 1 | 2026-07-30 |
| Ja*** | 1 | 2026-07-20 |
| Br*** | 1 | 2026-07-07 |
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