Stbl3 (containing pLV3-RSV-U6-sgRNA1-EF1a-iRFP720-P2A-Hyg plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold at 30°C, bacteria at 37°C) with rapid shaking to dissolve; ② Wipe the outer surface of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, pipette out all the dissolved liquid, and spread onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24 h before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50 mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37°C on a shaking incubator; ⑥ According to experimental needs, pipette an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing