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Sunlong Medical™ Rat IL-2 ELISA Kit
Sunlong Medical™ Rat IL-2 ELISA Kit
IL2 elisa kit | interleukin 2 elisa kit | IL-2 | interleukin-2 | T-cell growth factor | TCGF
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  • NO.:EL0038Ra
    Species:Rat
    Assay range:31.25pg/mL-2000pg/mL
    Sensitivity:1.38 pg/mL
    Sample Volume:Serum | plasma;20 μL;Cell culture supernatant;100 μL
    Assay type:Sandwich Method
    Validity:Two Years
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Citations

Cat NO.:
EL0038Ra
Product:
Sunlong Medical™ Rat IL-2 ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
85% - 110%
Mean Spike Recovery:
1.02
CV of Intra plate:
3.5 % - 6.8 %
CV of Inter plate:
5.5 % - 8.2 %
NCBI_Gene:
116562
UniProtKB:
P17108

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-2 monoclonal antibody
Rat IL-2 freeze-dried standard
IL-2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-2 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Biomaterials Science 2023-05-02
 
MATERIALS & DESIGN 2023-10-18
 
BIOMEDICINE & PHARMACOTHERAPY 2023-08-17
 
Acta Pharmaceutica Sinica B 2023-06-20
 
JOURNAL OF ULTRASOUND IN MEDICINE 2023-03-14

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