DH5α (containing pCRE-miniP-Fluc-hPEST-Neo plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold at 30°C, bacteria at 37°C) with rapid shaking until dissolved; ② Disinfect the outer surface of the cryovial with an alcohol swab, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire dissolved suspension, and spread it onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24 h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic Recommended Medium, then culture overnight at 37°C on a shaker; ⑥ According to experimental requirements, aspirate an appropriate amount of the bacterial culture for plasmid extraction;
Sharing Mode	Public Welfare Sharing

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