DH5α (containing pET-43.1a plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (mold at 30℃, bacteria at 37℃) and shake rapidly to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol swab, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the dissolved liquid, and dispense it onto two corresponding antibiotic-resistant plates, about 200μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24h and observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37℃ on a shaking incubator; ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing

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