Stbl3 (containing pLX304-FLAG-MCS-NES plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (molds at 30°C, bacteria at 37°C) and shake rapidly to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol swab, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, draw out all the dissolved liquid, and spread it onto 2 plates with the corresponding antibiotic, approximately 200 μL per plate; ④ Incubate the antibiotic plates under the specified conditions above for 18-24 h and then observe (do not seal or wrap the plates tightly); ⑤ Pick a single colony and inoculate it into 50 mL liquid LB antibiotic Recommended Medium, then culture overnight on a shaker at 37°C; ⑥ According to experimental needs, draw an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing