Stbl3 (containing pLV3-CMV-4×Mito-EF1a-CopGFP-Puro plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	1. Thaw the cryovial in a water bath (mold at 30°C, bacteria at 37°C) and shake rapidly to dissolve; 2. Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; 3. Unscrew the cap and aspirate all the dissolved solution, then plate onto 2 corresponding antibiotic plates, approximately 200 μL per plate; 4. Incubate the antibiotic plates under the specified conditions above for 18-24 h, then observe (do not seal or wrap the plates); 5. Pick a single colony and inoculate into 50 mL of liquid LB antibiotic Recommended Medium, culture overnight at 37°C on a shaker; 6. According to experimental requirements, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing