P3HR-1 Cell-Specific Culture Medium - Basic Information

Culture Medium	80% RPMI-1640 + 20% Heat-Inactivated FBS
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear, transparent, light yellow
Procedure	① Preparation: Sterilize the pipette, 50mL centrifuge tube, 100mL serum bottle, and clean bench with UV light for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Mixing: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically pour 20% heat-inactivated FBS into the 80% basal medium. ③ Sealing: Seal the culture medium bottle with parafilm, attach the corresponding product label, and store the medium back in the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and irradiate with UV light for 30 minutes.