W6/32 Cell-Specific Culture Medium - Basic Information

Culture Medium	89% DMEM-H + 10% FBS + 1% NEAA
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent
Procedure	① Preparation: Place pipettes, 50 mL centrifuge tubes, 100 mL serum bottles, and the clean bench under UV light for 20 minutes; pre-warm the basal medium in a 37°C constant temperature water bath; ② Preparation: Sterilize the openings of the basal medium bottle and serum bottle with a flame, then pour 10 mL of serum and 1 mL of NEAA into 89 mL of basal medium; ③ Sealing: Seal the culture medium bottle opening with parafilm, attach the corresponding product label, and store the culture medium back in the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV irradiate for 30 minutes;