P19 Cell-Specific Culture Medium - Basic Information

Culture Medium	90% MEM-α + 7.5% CS + 2.5% FBS
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent, red
Procedure	① Preparation: UV irradiate pipettes, 50mL centrifuge tubes, 100mL serum bottles, and the laminar flow hood for 20 minutes; pre-warm the basal medium in a 37°C water bath; ② Mixing: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then pour 7.5% CS and 2.5% FBS into the 90% MEM-α by suspending them; ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and return the medium to a 4°C refrigerator. Wipe the laminar flow hood with alcohol, extinguish the flame, and UV irradiate for 30 minutes;

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