BT Cell Specialized Medium - Basic Information

Medium	90% DMEM-H (1.5g/L NaHCO₃) + 10% HS
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent
Procedure	① Preparation: Sterilize the pipette, 50mL centrifuge tube, 100mL serum bottle, and biosafety cabinet with UV light for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Mixing: Flame-sterilize the openings of the basal medium bottle and serum bottle, then aseptically pour 10mL of serum into 90mL of basal medium. ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and store the culture medium back in the 4°C refrigerator. Wipe down the biosafety cabinet with alcohol, extinguish the flame, and UV-irradiate for 30 minutes.