Various cyanine dyes have been used to label biomolecules for fluorescence imaging and other fluorescence-based biochemical assays. They are widely used to label peptides，proteins，and oligonucleotides，among others. Cy5 dye is one of the common red fluorophores. These versatile fluorophores can tolerate a pH range of 3-10 and are used in a variety of applications at biologically relevant pH. These dyes are also resistant to DMSO and are photostable to transfer from storage to analysis without loss of performance. Sulfo-Cyanine 5 is an alternative dye primarily used to replace Cy5 developed by GE Healthcare for labeling peptides and oligonucleotides.

 

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