Monochlorodiamine (mBCl) is essentially non-fluorescent until it reacts with a thiol group. It readily reacts with several low molecular weight thiols，including cysteine，glutathione，N-acetylcysteine，mercaptopurine，peptides，and plasma thiols. The glutathione conjugate of mBCl has an absorption/emission maximum of ~394/490 nm.

 

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