This product is a cell-permeable X-rosamine derivative that can specifically label biologically active mitochondria in cells and detect mitochondrial membrane potential. This product is a high-purity probe, which is compatible with subsequent cell fixation and permeabilization, but after staining, the fluorescence intensity will be reduced to a certain extent. This product is an oxidized red fluorescent dye that can be passively transported across the cell membrane by simple incubation with cells, and biologically active mitochondria are specifically labeled with the help of the weakly sulfhydryl-reactive chloromethyl functional groups contained in this probe. This probe contains chloromethyl groups, which can react with sulfhydryl groups of mitochondrial proteins and covalently link, so when subsequent experiments are treated with aldehyde fixatives such as paraformaldehyde or formaldehyde, as well as cell-permeable detergent Triton X-100, the fluorescent labeling of mitochondria will not disappear, but may decrease to a certain extent (about 2-10 times). Since the excitation and emission spectra of this fluorescent probe overlap less than the common green fluorescent probe, it is suitable for fluorescence double-label experiments.
It can be used for mitochondrial fluorescence staining of living cells, but is not suitable for staining cells or tissues after fixation; However, after labeling mitochondria with this product, it can be fixed and permeabilized in the future, and stained with other probes or immunofluorescence staining, but after staining, the fluorescence intensity will be reduced to a certain extent. Moreover, the effects of fixation and permeabilization will be different for different cells, so it is recommended to perform certain tests on fixation and permeabilization reagents and conditions.
This product contains only a trace amount of powder, please centrifuge for a few seconds before use to allow the trace powder to settle to the bottom of the tube. Fluorochromes have quenching problems, please try to avoid light to slow down the fluorescence quenching.
This product can be used for fluorescent staining in common multi-well plates or dishes, as well as on coverslips or glass slides.

 

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