中文名称（Chinese Synonym）： 二苯甲亚胺, 三氯化氢 2-(4-乙基苯基)-5-(4-甲基-1-哌嗪基)-2,5-二-1H-苯并咪唑rn

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rn 英文名称（English Synonym） ：2’-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole, trihydrochloridern

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rn 说明：荧光色素；可与弗伦德红白血病细胞可逆地结合；用于DNA、染色体和核酸的染色；荧光染色法和免疫荧光技术。可用于荧光显微镜或流式细胞仪。rn

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rn 物理性状：溶于水，参考浓度为49.00 - 51.00 mg/ml。rn

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rn 荧光光谱（Fluorescence Spectral）：Hoechst 33342的Ex/Em=346/460; Hoechst33342-核酸的Ex/Em=350/461. 荧光可用氙气灯，泵弧灯或者UV激光激发；可用DAPI滤光器、蓝色GFP滤光器、Alexa Fluor 350滤光器等检测。rn

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rn Hoechst 33342 是一种可以穿透细胞膜的蓝色荧光染料，它在嵌入双链 DNA 后释放强烈的蓝色荧光，对细胞的毒性较低。Hoechst 33342 常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。也可以用于普通的细胞核染色，或常规的DNA染色。rn

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rn 用于细胞核染色时，推荐的Hoechst 33342工作浓度为0.5-10μg/ml。如需特定浓度或者经浓度优化的产品，可选购Hoechst 33342染液(1mg/ml)（Cat No.DR0806）、Hoechst 33342染液(即用型)（Cat No. DR0582）。rn

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rn 注意事项: rn

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rn (1)用于细胞实验，溶解后，须用一次性针头滤器过滤除菌。rn

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rn (2)为了您的安全和健康，请穿实验服并戴一次性手套操作。rn

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rn   备注：产品信息可能会有优化升级。请以实际标签信息为准。rn

 

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