Product Introduction:

Alcian is a kind of copper titanium cyan conjugated dye, which was originally used for textile fiber dyeing. This kind of cationic dye can combine with acidic groups, that is, alcian blue forms an insoluble blue complex with anionic groups such as carboxyl and sulfate.

Hyaluronidase combined with alcian blue staining is used in the Hyaluronic Acid Stain Kit. The principle is that hyaluronidase can cleave the glycosidic bond of hyaluronic acid and chondroitin sulfate. Before alcian blue staining, the sample is pretreated with hyaluronidase. Compared with the untreated section, if the treated section is not stained, it indicates that there are hyaluronic acid and chondroitin sulfate in the tissue If there is no effect, the tissue does not contain hyaluronic acid and chondroitin sulfate.

 

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

 

Experiment picture

Paraffin sections of mouse knee joints

Hyaluronidase digestion treatment; few pale blue-green areas observed in cartilage

,

Experiment picture

Paraffin sections of mouse knee joints

Without digestion treatment, the cartilage and hyaluronic acid areas are stained dark blue-green

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