Alamar Blue reagent provides a simple, rapid, reliable and safe method for the detection of cell proliferation and cytotoxicity, which is suitable for high-throughput detection experiments. The main component of the assay reagent is a REDOX indicator. In the oxidation state,it showed a purplish-blue non-fluorescence, while in the reduction state, it changed to a reduction product with pink or red fluorescence, with an absorption peak of 530-560nm and a scattering peak of 590nm.

During cell proliferation, the ratio of NADPH/NADP, FADH/FAD, FMNH/FMN, and NADH/NAD in the cells increased and are in a reducing environment. The ingested dye is reduced by these metabolic intermediates and cytochromes and released outside the cell and dissolved in the medium, changing the medium from a non-fluorescent indigo blue to a fluorescent pink. Finally, a common spectrophotometer or a fluorescence photometer is used for detection, and the absorbance and fluorescence intensity are proportional to the number of viable cells.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Experiment picture

Cells inoculated at different densities exhibit different colors under the same reaction conditions.

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