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Trypan Blue, is a cell reactive dye, which is often used to detect the integrity of cell membrane and the survival rate of cells. It is one of the most commonly used dead cell identification methods in tissue and cell culture. Normal living cells have intact cell membrane structure and can reject trypan blue, so the cells will not be stained blue. However, cells with loss of activity or incomplete cell membrane can be stained blue by trypan blue. It is generally considered that the cell has died when the integrity of the cell membrane is lost.
Trypan blue can be phagocytosed by macrophages, so it can be used as an in vivo stain for macrophages. Trypan blue exclusion is also observed for apoptotic bodies. After trypan blue staining, a more precise quantification of cell viability can be obtained by direct counting under the microscope or by counting after being photographed under the microscope. The staining time is only 3-5 minutes and the procedure is simple.
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