Assay Principle

This kit employs double antibody sandwich ELISA technology: Capture Antibody is coated on the microplate to capture PARK7 from samples and standards. After washing, biotin-labeled Detection Antibody is added and incubated, followed by washing to form a "Capture Antibody-

Antigen-Detection Antibody" immune-complex. Subsequently, Streptavidin-Horseradish Peroxidase (SA-HRP) is added and incubated. After incubation and washing, TMB Substrate is added for color development. If the target analyte is present in the sample, a blue color develops. Stop Solution is then added to terminate the reaction. During the assay, unbound components are washed away. The Optical Density (OD) is measured at 450 nm using a microplate reader. The intensity of the color is proportional to the PARK7 concentration in the sample, and the concentration is calculated by plotting a standard curve.

Typical Data

The following data and curve are for reference only. The experimenter must establish a standard curve based on their own experimental data.

 

Standard Conc. (ng/mL)	100	50	25	12.5	6.25	3.125	1.563	0
OD Value	2.702	1.533	0.795	0.421	0.229	0.134	0.086	0.036
Corrected OD Value	2.666	1.497	0.759	0.385	0.193	0.098	0.05	0

 

Sensitivity

The minimum detectable concentration (sensitivity) of Human PARK7, determined by testing samples, is 0.78ng/mL.

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