Typical Data

The following data and curve are for reference only. The experimenter must establish a standard curve based on their own experimental data.

Standard Conc. (ng/mL)	10	5	2.5	1.25	0.625	0.313	0
OD Value	2.705	1.535	0.795	0.421	0.221	0.133	0.037
Corrected OD Value	2.668	1.498	0.758	0.384	0.184	0.096	0

Recovery

Recovery was tested by spiking known concentrations of Rat LEP into different sample matrices. The recovery range and average recovery are shown below.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=8)	89-99	96
Plasma (n=8)	95-103	98
Cultured Cells (n=8)	96-115	103

Linearity

High concentration Rat LEP was spiked into samples and then serially diluted 2-fold, 4-fold, 8-fold, and 16-fold within the range of the standard curve to assess linearity. Recovery rates and average recovery are shown below.

Dilution		Serum (n=4)	Cultured Cells (n=4)
1:2	Recovery Range (%)	94-112	91-105
	Avg. Recovery (%)	99	101
1:4	Recovery Range (%)	99-114	97-112
	Avg. Recovery (%)	101	107
1:8	Recovery Range (%)	102-119	99-114
	Avg. Recovery (%)	106	111
1:16	Recovery Range (%)	109-119	106-120
	Avg. Recovery (%)	110	118

 

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