DESCRIPTION

Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be an important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin's disease.

PRECISION

Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,214.0	80.3	3.6
2	8	509.1	23.7	4.7
3	8	272.5	14.6	5.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,164.8	123.9	5.7
2	16	513.1	23.6	4.6
3	16	273.2	17.9	6.6

RECOVERY

The recovery of human IL-13 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Dilution	Average % of Expected	Range (%)
Human plasma	1:2	77	72-84
Cell culture supernatant	1:2	97	95-99
Cell culture supernatant	1:4	101	94-108

LINEARITY

To assess the linearity of the assay, human plasma samples were spiked with high concentrations of human IL-13 and diluted with the appropriate Sample Diluent (Sample Diluent PT 4B1-ef) to produce samples with values within the dynamic range of the assay. Cell culture supernatant samples were diluted with the appropriate Sample Diluent (Sample Diluent PT 4B1) to produce samples with values within the dynamic range of the assay.

	Human plasma (Sample Diluent PT 4B1-ef)		Cell culture supernatant (Sample Diluent PT 4B1)
	Average % of Expected	Range (%)	Average % of Expected	Range (%)
1:2	79	78-80	100	-
1:4	87	85-90	102	101-103
1:8	81	77-84	109	108-109
1:16	89	84-95	104	98-110

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human IL-13 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)