Summary:

 

Purpose

Our is to assay Na+/K+ATPase levels in Fish serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to Na+/K+ATPase, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Na+/K+ATPase is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain Na+/K+ATPase and HRP conjugated Na+/K+ATPase antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of Na+/K+ATPase is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS0115FI is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0U/ml	0.040	0.0450
		0.050
S1	4.8U/ml	1.958	1.9555
		1.953
S2	3.2U/ml	1.308	1.3090
		1.310
S3	1.6U/ml	0.698	0.7045
		0.711
S4	0.8U/ml	0.397	0.3935
		0.390
S5	0.4U/ml	0.209	0.2075
		0.206

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