Aliases:	PRKDC; Protein Kinase, DNA-Activated, Catalytic Subunit; DNPK1; P460; DNA-Dependent Protein Kinase Catalytic Subunit; DNA-PKcs; DNA-PKC; DNAPK; XRCC7; HYRC1; P350; HYRC; Protein Kinase, DNA-Activated, Catalytic Polypeptide; DNA-PK Catalytic Subunit; EC 2.7.11.1; DNAPKc; Hyper-Radiosensitivity Of Murine Scid Mutation, Complementing 1; DNA-PKCS; DNAPKC; IMD26
Background:	UniProt Entry: P78527;NCBI Gene Entry: 5591
Application Information	Molecular Weight: Predicted, 469 kDa, observed, 469 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2055 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human DNA-PKcs
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of DNA PKcs expression in HepG2 cells using DNA PKcs antibody 1:2,000. Green, isotype control; red, DNA PKcs.

Immunocytochemical staining of HepG2 cells with DNA PKcs antibody 1:1,000. Nuclei were stained blue with DAPI; DNA PKcs was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-DNA PKcs antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-DNA PKcs antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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