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Human Gastric Intrinsic Factor (GIF)ELISA Kit
Human Gastric Intrinsic Factor (GIF)ELISA Kit
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Regular members: $374.4
  • NO.:SL4517Hu
    Assay range:31.2pg/ml-2000pg/ml
    Sensitivity:5.6pg/ml
    Standard:2700pg/ml
    Sample Volume:10ul
    Wavelength:450nm
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Drug Names

Generic Name: Human Gastric Intrinsic Factor (GIF)ELISA Kit

Purpose

Our Human Gastric Intrinsic Factor (GIF)ELISA kit is to assay GIF levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GIF. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GIF is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GIF and HRP conjugated GIF antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GIF. You can calculate the concentration of GIF in the samples by comparing the OD of the samples to the standard curve.

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