Summary:

 

Purpose

 

Our Quick Step Mouse soluble P-selectin,sP-selectin ELISA Kit is to assay sP-selectin levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to sP-selectin. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for sP-selectin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain sP-selectin and HRP conjugated sP-selectin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of sP-selectin. You can calculate the concentration of sP-selectin in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0505Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.048	0.0500
		0.052
S1	300pg/ml	1.939	1.9455
		1.952
S2	200pg/ml	1.372	1.3865
		1.401
S3	100pg/ml	0.722	0.7200
		0.718
S4	50pg/ml	0.411	0.4035
		0.396
S5	25pg/ml	0.201	0.2065
		0.212

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