Aliases:	AKT1S1; AKT1 Substrate 1; PRAS40; 40 KDa Proline-Rich AKT Substrate; Proline-Rich AKT1 Substrate 1; MGC2865; Lobe; Proline-Rich Akt Substrate, 40 KDa; AKT1 Substrate 1 (Proline-Rich); AKT1 Substrate 1 (Proline Rich); LOBE
Background:	UniProt Entry: Q96B36;NCBI Gene Entry: 84335
Application Information	Molecular Weight: Predicted, 27 kDa, observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3855 Species Reactivity: Human, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-PRAS40 (T246)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Phospho-PRAS40 (T246) expression in HeLa cells using Phospho-PRAS40 (T246) antibody 1:2,000. Green, isotype control; red, Phospho-PRAS40 (T246).

Immunocytochemical staining of HeLa cells with Phospho-PRAS40 (T246) antibody 1:1,000. Nuclei were stained blue with DAPI; Phospho-PRAS40 (T246) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-PRAS40 (T246) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Phospho-PRAS40 (T246) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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