Aliases:	E2F1; E2F Transcription Factor 1; RBBP3; RBP3; Retinoblastoma-Binding Protein 3 (E2F-Like); Retinoblastoma-Associated Protein 1; Retinoblastoma-Binding Protein 3; Transcription Factor E2F1; PRB-Binding Protein E2F-1; RBAP-1; RBBP-3; E2F-1; PBR3; RBAP1
Background:	UniProt Entry: Q01094;NCBI Gene Entry: 1869
Application Information	Molecular Weight: Predicted, 47 kDa, observed, 65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1505 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human E2F1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of E2F transcription factor 1 expression in C2C12 cells using E2F transcription factor 1 antibody 1:2,000. Green, isotype control; red, E2F transcription factor 1.

Immunocytochemical staining of C2C12 cells with E2F transcription factor 1 antibody 1:1,000. Nuclei were stained blue with DAPI; E2F transcription factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-E2F transcription factor 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-E2F transcription factor 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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