Aliases:	EIF2S1; Eukaryotic Translation Initiation Factor 2 Subunit Alpha; EIF-2alpha; EIF2A; Eukaryotic Translation Initiation Factor 2, Subunit 1 Alpha, 35kDa; Eukaryotic Translation Initiation Factor 2 Subunit 1; EIF-2-Alpha; EIF2-Alpha; EIF-2A; EIF2; Eukaryotic Translation Initiation Factor 2, Subunit 1 (Alpha, 35kD ); EIF-2
Background:	UniProt Entry: P05198;NCBI Gene Entry: 1965
Application Information	Molecular Weight: Predicted, 36 kDa, observed, 36 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3905 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-eIF2 alpha (Ser51)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-phospho-eIF2 alpha (Ser51) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using FeQTM ECL Substrate Kit .

Western blotting analysis using anti-phospho-eIF2 alpha (Ser51) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using Plus ECL Super Kit 1:2,000. Green, isotype control; red, phospho-eIF2 alpha (Ser51).

Immunocytochemical staining of HT-1080 cells with anti-Phospho-eIF2 alpha (Ser51) antibody 1:1,000 . Nuclei were stained blue with DAPI; Phospho-eIF2 alpha (Ser51) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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