Aliases:	GLI Family Zinc Finger 3; PAP-A; PAPA1; PPDIV; PAPA; PAPB; ACLS; Glioma-Associated Oncogene Family Zinc Finger 3; GLI-Kruppel Family Member GLI3;Transcriptional Activator GLI3; Zinc Finger Protein GLI3; Oncogene GLI3; GLI3-190; GLI3FL; GCPS; PHS; Greig Cephalopolysyndactyly Syndrome; GLI3 Full-Length Protein; GLI3 Form Of 190 KDa; DNA-Binding Protein
Background:	UniProt Entry: P10071;NCBI Gene Entry: 2737
Application Information	Molecular Weight: Predicted, 170 kDa, observed, 190 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6105 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Gli3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-gli3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using FeQTM ECL Substrate Kit . GLI3, GLI Family Zinc Finger 3.

Flow cytometric analysis of gli3 expression in HepG2 cells using anti-gli3 antibody 1:2,000. Green, isotype control; red, gli3.

Immunocytochemical staining of HepG2 cells with anti-gli3 antibody 1:1,000. Nuclei were stained blue with DAPI; Gli3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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